Home > Seminars > Purification of DNA Therapeutics: New Opportunities for Membrane Technology

Purification of DNA Therapeutics: New Opportunities for Membrane Technology

Start:

4/16/2013 at 3:30PM

End:

4/16/2013 at 4:45PM

Location:

140 DeBartolo Hall

Host:

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William Phillip

William Phillip

VIEW FULL PROFILE Email: wphillip@nd.edu
Phone: 574-631-2708
Website: http://www3.nd.edu/~waterlab/index.html
Office: 205F McCourtney Hall

Affiliations

College of Engineering Associate Professor
Chemical separations are essential to the production of freshwater and the generation of fuels. Traditionally energy-intensive thermal processes have been used to effect these separations. Membrane separations, an alternative to thermally-driven separations, are gaining increased attention because ...
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There is growing interest in using plasmid DNA for gene therapy and DNA-based vaccines. Existing methods for the purification of plasmid DNA are inadequate for large-scale commercial production; thus, there is a critical need for the development of new separations technology specifically targeted for plasmid purification. This talk examines the possibility of using membrane ultrafiltration for the purification of supercoiled plasmid DNA, including removal of host cell-related impurities and product-related isoforms. Plasmid transmission during ultrafiltration was a strong function of filtrate flux and ionic strength. This flux-dependence was described using a model that accounts for the elongation of the plasmid associated with the converging flow into the membrane pores. Plasmid sieving coefficients were in good agreement with model calculations over a wide range of conditions, providing an appropriate framework for analysis of plasmid ultrafiltration data. Transmission of the open circular DNA was significantly less than that of the supercoiled plasmid, while transmission of the linear DNA was considerably enhanced due to differences in the conformational flexibility of these DNA isoforms. These data were used to identify appropriate conditions for purification of the desired supercoiled isoform. The results clearly demonstrate the potential application of ultrafiltration for the commercial-scale purification of plasmid DNA.

Seminar Speaker:

Dr. Andrew Zydney

Dr. Andrew Zydney

The Pennsylvania State University

Dr. Andrew L. Zydney is currently Department Head and Walter L. Robb Family Endowed Chair in the Department of Chemical Engineering at The Pennsylvania State University. Professor Zydney received his Ph.D. in Chemical Engineering from M.I.T. in 1985, and he was a faculty member in the Chemical Engineering Department at the University of Delaware from 1985 - 2001. Professor Zydney's research is focused on membrane science and technology, with a particular emphasis on bioseparations and the purification of high value biological products. He has published more than 170 articles on these topics, including invited contributions to the Encyclopedia of Bioprocess Technology and the Handbook of Biomedical Engineering. Professor Zydney is the Editor-in-Chief of the Journal of Membrane Science, and he serves on the Editorial Boards for Separation and Purification Reviews, Separation Science and Technology, Journal of Colloid and Interface Science, and Biotechnology and Bioengineering. He served as President of the North American Membrane Society in 2002 - 2003, he was elected a fellow of the American Institute of Medical and Biological Engineers in 1998, he received the Excellence in Teaching Award from the University of Delaware in 1994, and he is a past recipient of the Distinguished Teacher Award (1999) and the Outstanding Young Faculty Award (1990) from the American Society of Engineering Education.

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